|
KMID : 0811720150190040383
|
|
Korean Journal of Physiology & Pharmacology
2015 Volume.19 No. 4 p.383 ~ p.388
|
|
|
Characteristics of K+ Outward Currents in the Cochlear Outer Hair Cells of Circling Mice within the First Postnatal Week
|
|
Ahn Ji-Woong
Kang Shin-Wook
Ahn Seung-Cheol
|
|
|
Abstract
|
|
|
|
K+ outward currents in the outer hair cells (OHCs) of circling mice (homozygous (cir/cir) mice), an animal model for human deafness (DFNB6 type), were investigated using a whole cell patch clamp technique. Littermate heterozygous (+/cir) mice of the same age (postnatal day (P) 0 ?P6) were used as controls. Similar slow rising K+ currents were observed in both genotypes, but their biophysical and pharmacological properties were quite different. The values of Vhalf for activation were significantly different in the heterozygous (+/cir) and homozygous (cir/cir) mice (－8.1±2.2 mV, heterozygous (+/cir) mice (n=7) and －17.2±4.2 mV, homozygous (cir/cir) mice (n=5)). The inactivation curve was expressed by a single first order Boltzmann equation in the homozygous (cir/cir) mice, while it was expressed by a sum of two first order Boltzmann equations in the heterozygous (+/cir) mice. The K+ current of homozygous (cir/cir) mice was more sensitive to TEA in the 1 to 10 mM range, while the 4-AP sensitivities were not different between the two genotypes. Removal of external Ca2+ did not affect the K+ currents in either genotype, indicating that the higher sensitivity of K+ current to TEA in the homozygous (cir/cir) mice was not due to an early expression of Ca2+ activated K+ channels. Our results suggest that the K+ outward current of developing homozygous (cir/cir) mice OHCs is different in both biophysical and pharmacological aspects than that of heterozygous (+/cir) mice.
|
|
|
KEYWORD
|
|
|
Circling mice, Deafness, DFNB6, K+ current, Outer hair cell
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|